Hotspot

rpl32-trnL-UAG

SSC · 108098-108797 · 700 bp

Back to Shivparvatia

Pi 0.0974
MarkerSeek score 84.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
700
variable_sites
61
pi
0.0974
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACGAAAAATAAGTACTCAAAACTTGG TCCTAAGAGCAGCGTGTCTA 30.8 50.0 56.9 57.9 718-770 bp 1.0 50.6
rpl32-trnL-UAG_p2 ACGAAAAATAAGTACTCAAAACTTGGA TCCTAAGAGCAGCGTGTCTA 29.6 50.0 58.1 57.9 718-770 bp 1.0 50.6
rpl32-trnL-UAG_p3 ACGAAAAATAAGTACTCAAAACTTGG AGCAGCGTGTCTACCAATTT 30.8 45.0 56.9 57.8 711-763 bp 1.0 50.6
rpl32-trnL-UAG_p4 ACGAAAAATAAGTACTCAAAACTTGG CCTAAGAGCAGCGTGTCTAC 30.8 55.0 56.9 57.8 717-769 bp 1.0 50.6
rpl32-trnL-UAG_p5 ACGAAAAATAAGTACTCAAAACTTGG CTAAGAGCAGCGTGTCTACC 30.8 55.0 56.9 57.8 716-768 bp 1.0 50.6