Hotspot

petA-psbJ

LSC · 58833-59686 · 854 bp

Back to Shivparvatia

Pi 0.0617
MarkerSeek score 77.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
854
variable_sites
45
pi
0.0617
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GTTCAATTGTCCGAAATGAA ATTCTGGATTGGGCTCATCG 35.0 50.0 52.4 57.7 794-907 bp 1.0 48.2
petA-psbJ_p2 TCAATTGTCCGAAATGAATTTCT ATTCTGGATTGGGCTCATCG 30.4 50.0 55.1 57.7 792-905 bp 1.0 48.2
petA-psbJ_p3 GTTCAATTGTCCGAAATGAATTTCT ATTCTGGATTGGGCTCATCG 32.0 50.0 57.1 57.7 794-907 bp 1.0 48.2
petA-psbJ_p4 GTTCAATTGTCCGAAATGAAT ATTCTGGATTGGGCTCATCG 33.3 50.0 53.1 57.7 794-907 bp 1.0 48.2
petA-psbJ_p5 GTTCAATTGTCCGAAATGAATT ATTCTGGATTGGGCTCATCG 31.8 50.0 53.9 57.7 794-907 bp 1.0 48.2