Hotspot

rps4-trnT-UGU

LSC · 43174-43351 · 178 bp

Back to Shivparvatia

Pi 0.071

MarkerSeek score 77.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 12
pi: 0.071
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GCCCCTAAACGGCGTATTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.9	57.9	290-291 bp	1.0	77.5
rps4-trnT-UGU_p2	GCCCCTAAACGGCGTATTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.9	57.8	258-259 bp	1.0	77.4
rps4-trnT-UGU_p3	GCCCCTAAACGGCGTATTT	CTCAGAGGTTAGAGCATCGC	52.6	55.0	58.2	57.9	290-291 bp	1.0	75.0
rps4-trnT-UGU_p4	GCCCCTAAACGGCGTATTTT	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.9	57.5	298-299 bp	1.0	74.4
rps4-trnT-UGU_p5	GCCCCTAAACGGCGTATTTT	GAATTTCTACGAGCCCGCTT	50.0	50.0	58.9	58.3	313 bp	0.5	50.0