Hotspot

atpF-atpH

LSC · 12557-13040 · 484 bp

Back to Shivparvatia

Pi 0.0729

MarkerSeek score 80.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 484
variable_sites: 32
pi: 0.0729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	CCGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	604-639 bp	1.0	82.3
atpF-atpH_p2	CCAGTGACCCAAGAAAACGA	GCCGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	605-640 bp	1.0	82.2
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTT	50.0	45.0	58.0	56.8	535-570 bp	1.0	78.8
atpF-atpH_p4	CCAGTGACCCAAGAAAACGA	TTGGTTGTAGCATTAGCCCT	50.0	45.0	58.0	56.8	536-571 bp	1.0	78.8
atpF-atpH_p5	CCAGTGACCCAAGAAAACGA	TGGTTGTAGCATTAGCCCTTT	50.0	42.9	58.0	57.6	535-570 bp	1.0	76.7