Hotspot

trnR-UCU-atpA

LSC · 9601-9698 · 98 bp

Back to Shivparvatia

Pi 0.102

MarkerSeek score 77.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 10
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TCAAGAACAGATGGAACGCT	47.6	45.0	57.3	57.2	163-210 bp	1.0	64.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TGGAACGCTTTCTACTTCAGG	47.6	47.6	57.3	58.0	152-199 bp	1.0	62.7
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TCAAGAACAGATGGAACGCTT	47.6	42.9	57.3	57.9	163-210 bp	1.0	61.7
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TTCAAGAACAGATGGAACGCT	47.6	42.9	57.3	57.9	164-211 bp	1.0	61.7
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	TCAAGAACAGATGGAACGCT	47.6	45.0	56.9	57.2	198-245 bp	1.0	60.5