Hotspot

rps16-trnQ-UUG

LSC · 5853-6537 · 685 bp

Back to Shivparvatia

Pi 0.0708
MarkerSeek score 79.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
685
variable_sites
43
pi
0.0708
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACGGATCATGTCCTT TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.9 57.3 722-783 bp 1.0 82.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 701-762 bp 1.0 82.2
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.2 57.3 712-773 bp 1.0 81.7
rps16-trnQ-UUG_p4 TGTCGAGTCAAGAGCATCCT TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.4 57.3 768-829 bp 1.0 79.4
rps16-trnQ-UUG_p5 ATGTCGAGTCAAGAGCATCC TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.4 57.3 769-830 bp 1.0 78.2