Hotspot

ycf3

LSC · 44453-46439 · 1987 bp

Back to Shibataea

Pi 0.000336

MarkerSeek score 45.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1987
variable_sites: 1
pi: 0.000336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGA	45.0	45.0	57.8	55.7	2130-2131 bp	1.0	60.4
ycf3_p2	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGA	45.0	45.0	57.8	55.7	2129-2130 bp	1.0	60.4
ycf3_p3	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGAT	45.0	42.9	57.8	56.3	2130-2131 bp	1.0	56.9
ycf3_p4	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGAT	45.0	42.9	57.8	56.3	2129-2130 bp	1.0	56.9
ycf3_p5	GATCAACAACCAAACCACCC	AGAGCATATGGATCGATCGA	50.0	45.0	57.2	55.7	2131-2132 bp	1.0	55.3