Hotspot

rps16

LSC · 4522-5608 · 1087 bp

Back to Shibataea

Pi 0.000613

MarkerSeek score 46.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1087
variable_sites: 1
pi: 0.000613
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGCACGACATAAATCCAGTCA	GGGTCAATTCCCTATACCACT	42.9	47.6	57.4	56.5	1255 bp	1.0	47.0
rps16_p2	AGCACGACATAAATCCAGTCA	GGGTCAATTCCCTATACCACTT	42.9	45.5	57.4	57.2	1255 bp	1.0	44.9
rps16_p3	AGCACGACATAAATCCAGTCA	TCCCTATACCACTTTGCAATGA	42.9	40.9	57.4	57.1	1247 bp	1.0	44.4
rps16_p4	ACGAGGGATACTAGTGATCCTC	GGGTCAATTCCCTATACCACT	50.0	47.6	58.0	56.5	1188 bp	1.0	43.7
rps16_p5	AGCACGACATAAATCCAGTCAA	GGGTCAATTCCCTATACCACT	40.9	47.6	58.1	56.5	1255 bp	1.0	43.4