Hotspot

petA

LSC · 62035-62997 · 963 bp

Back to Shibataea

Pi 0.000692

MarkerSeek score 46.70

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.000692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	ATTCTGGGATCTGCGATTGG	GCCAATAAATCGCGGCTTTT	50.0	45.0	57.7	58.1	1030 bp	1.0	77.3
petA_p2	ATTCTGGGATCTGCGATTGG	CAATCGCCAATAAATCGCGG	50.0	50.0	57.7	58.3	1035 bp	1.0	75.3
petA_p3	ATTCTGGGATCTGCGATTGG	AATCGCGGCTTTTTACCAAC	50.0	45.0	57.7	57.7	1023 bp	1.0	75.2
petA_p4	TCTGGGATCTGCGATTGGAT	GCCAATAAATCGCGGCTTTT	50.0	45.0	58.6	58.1	1028 bp	1.0	74.9
petA_p5	ATTCTGGGATCTGCGATTGG	AGCAATCGCCAATAAATCGC	50.0	45.0	57.7	57.6	1037 bp	1.0	74.7