Hotspot

rbcL-psaI

LSC · 58438-59686 · 1249 bp

Back to Shibataea

Pi 0.00374

MarkerSeek score 61.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1249
variable_sites: 7
pi: 0.00374
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1388 bp	1.0	78.8
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1394 bp	1.0	78.2
rbcL-psaI_p3	TCGAGTTCGATCCGGTAGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.7	58.0	1354 bp	1.0	78.2
rbcL-psaI_p4	TCGAGTTCGATCCGGTAGAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.7	57.9	1360 bp	1.0	77.6
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1365 bp	1.0	77.4