Hotspot

trnfM-CAU-trnT-GGU

LSC · 13584-16352 · 2769 bp

Back to Shibataea

Pi 0.000241

MarkerSeek score 57.20

Primer available yes

Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name: trnfM-CAU-trnT-GGU
feature_type: igs
region: LSC
length_bp: 2769
variable_sites: 1
pi: 0.000241
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnT-GGU_p1	CCTCAAGGTTATGAGCCTCG	CCATGGCATTACTCTACCACC	55.0	52.4	57.8	58.1	2897-2917 bp	1.0	69.6
trnfM-CAU-trnT-GGU_p2	CCTCGTGAGCTACCAAACTG	CCATGGCATTACTCTACCACC	55.0	52.4	58.3	58.1	2882-2902 bp	1.0	69.2
trnfM-CAU-trnT-GGU_p3	CCTCAAGGTTATGAGCCTCG	GACTTACGTCTTACCATGGCA	55.0	47.6	57.8	57.8	2910-2930 bp	1.0	68.9
trnfM-CAU-trnT-GGU_p4	CCTCAAGGTTATGAGCCTCG	TGACTTACGTCTTACCATGGC	55.0	47.6	57.8	57.8	2911-2931 bp	1.0	68.9
trnfM-CAU-trnT-GGU_p5	CCTCGTGAGCTACCAAACTG	GACTTACGTCTTACCATGGCA	55.0	47.6	58.3	57.8	2895-2915 bp	1.0	68.5