Hotspot

matK-trnK-UUU

LSC · 3232-3927 · 696 bp

Back to Shibataea

Pi 0.000959
MarkerSeek score 59.50
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
696
variable_sites
1
pi
0.000959
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 47.6 50.0 58.3 57.8 864-865 bp 1.0 68.4
matK-trnK-UUU_p2 ACTCCTGAAAGAGAAGTGGG CGTGCGGTGTAATTCCATTG 50.0 50.0 56.5 57.8 864-865 bp 1.0 66.5
matK-trnK-UUU_p3 CTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 50.0 50.0 56.5 57.8 863-864 bp 1.0 66.5
matK-trnK-UUU_p4 ACTCCTGAAAGAGAAGTGGGT TGGGTTGCTAACTCAATGGT 47.6 45.0 58.3 57.0 805-806 bp 1.0 62.0
matK-trnK-UUU_p5 ACTCCTGAAAGAGAAGTGGGTA CGTGCGGTGTAATTCCATTG 45.5 50.0 58.1 57.8 864-865 bp 1.0 61.7