Hotspot

rps15-trnN-GUU

IRa · 117201-118776 · 1576 bp

Back to Setaria

Pi 0.00106

MarkerSeek score 60.20

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1576
variable_sites: 3
pi: 0.00106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	AATGTGAAGCAAGTCTCCGT	ACGACCAGTCAGTTAACAGC	45.0	50.0	57.4	57.8	1704-1716 bp	1.0	75.1
rps15-trnN-GUU_p2	AATGTGAAGCAAGTCTCCGT	AGGATTCGAACCTACGACCA	45.0	50.0	57.4	58.2	1717-1729 bp	1.0	75.0
rps15-trnN-GUU_p3	AATGTGAAGCAAGTCTCCGT	GGATTCGAACCTACGACCAG	45.0	55.0	57.4	57.8	1716-1728 bp	1.0	74.6
rps15-trnN-GUU_p4	AATGTGAAGCAAGTCTCCGT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.4	57.7	1725-1737 bp	1.0	73.7
rps15-trnN-GUU_p5	AATGTGAAGCAAGTCTCCGT	TTCGAACCTACGACCAGTCA	45.0	50.0	57.4	58.4	1713-1725 bp	1.0	73.2