Hotspot

trnN-GUU-rps15

IRb · 102408-103983 · 1576 bp

Back to Setaria

Pi 0.00106

MarkerSeek score 60.20

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 1576
variable_sites: 3
pi: 0.00106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	ACGACCAGTCAGTTAACAGC	AATGTGAAGCAAGTCTCCGT	50.0	45.0	57.8	57.4	1704-1716 bp	1.0	75.1
trnN-GUU-rps15_p2	AGGATTCGAACCTACGACCA	AATGTGAAGCAAGTCTCCGT	50.0	45.0	58.2	57.4	1717-1729 bp	1.0	75.0
trnN-GUU-rps15_p3	GGATTCGAACCTACGACCAG	AATGTGAAGCAAGTCTCCGT	55.0	45.0	57.8	57.4	1716-1728 bp	1.0	74.6
trnN-GUU-rps15_p4	CCCCAAGTAGGATTCGAACC	AATGTGAAGCAAGTCTCCGT	55.0	45.0	57.7	57.4	1725-1737 bp	1.0	73.7
trnN-GUU-rps15_p5	TTCGAACCTACGACCAGTCA	AATGTGAAGCAAGTCTCCGT	50.0	45.0	58.4	57.4	1713-1725 bp	1.0	73.2