Hotspot

rbcL-psaI

LSC · 58103-58981 · 879 bp

Back to Setaria

Pi 0.0177

MarkerSeek score 62.30

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 30
pi: 0.0177
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCGATGGATACCCTATAG	AAGGAAGCCATTGCAATTGC	45.0	45.0	53.5	57.9	888-1016 bp	1.0	49.6
rbcL-psaI_p2	AAGCGATGGATACCCTATAG	GCCATTGCAATTGCTGGAAA	45.0	45.0	53.5	58.2	882-1010 bp	1.0	48.9
rbcL-psaI_p3	AAGCGATGGATACCCTATAG	GAAGCCATTGCAATTGCTGG	45.0	50.0	53.5	58.6	885-1013 bp	1.0	46.5
rbcL-psaI_p4	AAGCGATGGATACCCTATAG	GGAAGCCATTGCAATTGCTG	45.0	50.0	53.5	58.6	886-1014 bp	1.0	46.5
rbcL-psaI_p5	AAGCGATGGATACCCTATAG	AGGAAGCCATTGCAATTGCT	45.0	45.0	53.5	58.7	887-1015 bp	1.0	46.5