Hotspot

ndhC-trnV-UAC

LSC · 52276-52888 · 613 bp

Back to Setaria

Pi 0.0155
MarkerSeek score 59.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
613
variable_sites
17
pi
0.0155
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 36.4 55.0 56.1 57.9 658-993 bp 1.0 50.0
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA CGGTTCGAATCCGTATAGCC 36.4 55.0 56.1 58.3 659-994 bp 1.0 48.1
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 38.1 55.0 54.6 57.9 657-992 bp 1.0 46.0
ndhC-trnV-UAC_p4 ATCATATTCGTGAAGCAGAAACA GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 659-994 bp 1.0 45.9
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.6 57.9 658-993 bp 1.0 45.9