Hotspot

ycf3-trnS-GGA

LSC · 46278-46857 · 580 bp

Back to Setaria

Pi 0.00606

MarkerSeek score 59.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 7
pi: 0.00606
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTGGCTACAATCGAGGAGGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.4	58.2	632-740 bp	1.0	80.1
ycf3-trnS-GGA_p2	TTGGCTACAATCGAGGAGGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.4	57.8	582-690 bp	1.0	79.9
ycf3-trnS-GGA_p3	GGCTACAATCGAGGAGGTCT	ACGGAAAGAGAGGGATTCGA	55.0	50.0	58.6	58.2	630-738 bp	1.0	78.8
ycf3-trnS-GGA_p4	GGCTACAATCGAGGAGGTCT	TTCCAATGCTACGCCTTGAA	55.0	45.0	58.6	57.8	580-688 bp	1.0	78.6
ycf3-trnS-GGA_p5	TTGGCTACAATCGAGGAGGT	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.4	58.5	584-692 bp	1.0	77.0