Hotspot

trnM-CAU-trnT-GGU

LSC · 16019-16023 · 5 bp

Back to Setaria

Pi 0.1
MarkerSeek score 60.30
Primer available yes
Label trnM-CAU-trnT-GGU

Feature Metadata

label_name
trnM-CAU-trnT-GGU
feature_type
igs
region
LSC
length_bp
5
variable_sites
1
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-trnT-GGU_p1 CTCAGTGGTAGAGTAATGCCA ACTCTACCACTAAGTGAAAAG 47.6 38.1 56.8 52.6 81 bp 1.0 41.0
trnM-CAU-trnT-GGU_p2 ACTCAGTGGTAGAGTAATGCC ACTCTACCACTAAGTGAAAAG 47.6 38.1 56.8 52.6 82 bp 1.0 41.0
trnM-CAU-trnT-GGU_p3 AGCCCTTTTAACTCAGTGGT ACTCTACCACTAAGTGAAAAG 45.0 38.1 56.7 52.6 92 bp 1.0 40.9
trnM-CAU-trnT-GGU_p4 AAGCCCTTTTAACTCAGTGGT ACTCTACCACTAAGTGAAAAG 42.9 38.1 57.4 52.6 93 bp 1.0 40.9
trnM-CAU-trnT-GGU_p5 AAAGCCCTTTTAACTCAGTGGT ACTCTACCACTAAGTGAAAAG 40.9 38.1 58.1 52.6 94 bp 1.0 40.9