Hotspot

psbK-psbI

LSC · 7447-7848 · 402 bp

Back to Setaria

Pi 0.015

MarkerSeek score 62.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 10
pi: 0.015
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	GGATTACGTCCTGGGTCATT	50.0	50.0	58.0	57.0	537-581 bp	1.0	77.2
psbK-psbI_p2	CAAGCCGCTGTAAGTTTTCG	GGATTACGTCCTGGGTCATT	50.0	50.0	58.0	57.0	482-526 bp	1.0	77.1
psbK-psbI_p3	AGCCGCTGTAAGTTTTCGAT	GGATTACGTCCTGGGTCATT	45.0	50.0	57.9	57.0	480-524 bp	1.0	76.8
psbK-psbI_p4	AAGCCGCTGTAAGTTTTCGA	GGATTACGTCCTGGGTCATT	45.0	50.0	58.1	57.0	481-525 bp	1.0	76.5
psbK-psbI_p5	ACCCGAAGCTTATGCCATTT	GGATTACGTCCTGGGTCATT	45.0	50.0	57.9	57.0	567-611 bp	1.0	76.2