Hotspot

rpl22

LSC · 84927-85490 · 564 bp

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Pi 0.0337

MarkerSeek score 68.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 564
variable_sites: 19
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGGGTGGTACCAAGTCTGAA	TGTTTCTAGTTCATTAATAGGAGGT	50.0	32.0	58.2	55.5	627 bp	1.0	42.4
rpl22_p2	TTGGGTGGTACCAAGTCTGA	TGTTTCTAGTTCATTAATAGGAGGT	50.0	32.0	58.2	55.5	628 bp	1.0	42.4
rpl22_p3	TGGGTGGTACCAAGTCTGAA	ATGTTTCTAGTTCATTAATAGGAGGT	50.0	30.8	58.2	56.0	628 bp	1.0	42.4
rpl22_p4	TTGGGTGGTACCAAGTCTGA	ATGTTTCTAGTTCATTAATAGGAGGT	50.0	30.8	58.2	56.0	629 bp	1.0	42.4
rpl22_p5	GTTGTGCAAACCAAAGGGAA	TGTTTCTAGTTCATTAATAGGAGGT	45.0	32.0	57.3	55.5	656 bp	1.0	42.3