Hotspot

ndhE-ndhG

SSC · 120213-120444 · 232 bp

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Pi 0.0487

MarkerSeek score 76.50

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 232
variable_sites: 11
pi: 0.0487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTTCGGCTCGTGACTAATCC	AATAGGTGCAATTGCCGTGG	50.0	50.0	57.7	59.2	339-340 bp	1.0	72.2
ndhE-ndhG_p2	TCGGCTCGTGACTAATCCAT	AATAGGTGCAATTGCCGTGG	50.0	50.0	58.6	59.2	337-338 bp	1.0	69.8
ndhE-ndhG_p3	TTTCGGCTCGTGACTAATCC	ATAGGTGCAATTGCCGTGG	50.0	52.6	57.7	58.5	338-339 bp	1.0	69.6
ndhE-ndhG_p4	TTTCGGCTCGTGACTAATCC	TAATAGGTGCAATTGCCGTG	50.0	45.0	57.7	56.5	340-341 bp	1.0	69.6
ndhE-ndhG_p5	GCACATAAGAGCCCGAACTA	AATAGGTGCAATTGCCGTGG	50.0	50.0	57.4	59.2	361-362 bp	1.0	69.5