Hotspot

ccsA-ndhD

SSC · 117473-117676 · 204 bp

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Pi 0.0914

MarkerSeek score 75.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 204
variable_sites: 18
pi: 0.0914
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AAGTGCTTCCCCCTTTTCTG	TTTTTGATTCTGGGCCACGA	50.0	45.0	58.0	58.0	710-717 bp	1.0	83.6
ccsA-ndhD_p2	AAGTGCTTCCCCCTTTTCTG	TTGCGTTACCAGGTATGAGC	50.0	50.0	58.0	58.0	914-921 bp	1.0	83.0
ccsA-ndhD_p3	ACCTGCTCTACAATCCCAGT	TTTTTGATTCTGGGCCACGA	50.0	45.0	58.0	58.0	932-939 bp	1.0	82.8
ccsA-ndhD_p4	AAGTGCTTCCCCCTTTTCTG	TGACGAAATGGGCGGAATAG	50.0	50.0	58.0	58.1	988-995 bp	1.0	82.6
ccsA-ndhD_p5	AAGTGCTTCCCCCTTTTCTG	TATCTTGACGAAATGGGCGG	50.0	50.0	58.0	58.1	993-1000 bp	1.0	82.5