Hotspot

rpl32-trnL-UAG

SSC · 115044-116276 · 1233 bp

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Pi 0.0272

MarkerSeek score 60.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1233
variable_sites: 33
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGTTGGAAAGACAAGGGAT	ACTGGGATTGTAGAGCAGGT	45.0	50.0	56.6	58.0	1972-1975 bp	1.0	71.9
rpl32-trnL-UAG_p2	ATGGTTGGAAAGACAAGGGA	AGCGTGTCTACCGATTTCAC	45.0	50.0	56.6	58.0	1378 bp	0.5	49.0
rpl32-trnL-UAG_p3	TGGTTGGAAAGACAAGGGAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	56.6	58.0	1377 bp	0.5	49.0
rpl32-trnL-UAG_p4	ATGGTTGGAAAGACAAGGGA	CAGCGTGTCTACCGATTTCA	45.0	50.0	56.6	58.0	1379 bp	0.5	49.0
rpl32-trnL-UAG_p5	TGGTTGGAAAGACAAGGGAT	CAGCGTGTCTACCGATTTCA	45.0	50.0	56.6	58.0	1378 bp	0.5	49.0