Hotspot

ndhF-rpl32

SSC · 113726-114875 · 1150 bp

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Pi 0.0355

MarkerSeek score 68.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1150
variable_sites: 40
pi: 0.0355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCATATACGCCGAAGATT	TGTCCGGTATCCCTTGTCTT	50.0	50.0	57.9	58.1	1342-1353 bp	1.0	82.8
ndhF-rpl32_p2	GCCCATATACGCCGAAGATT	TTGTCCGGTATCCCTTGTCT	50.0	50.0	57.9	58.1	1343-1354 bp	1.0	82.8
ndhF-rpl32_p3	AGCAATAGGTCGTGTGAACC	TGTCCGGTATCCCTTGTCTT	50.0	50.0	57.9	58.1	1733-1744 bp	1.0	82.2
ndhF-rpl32_p4	AGCAATAGGTCGTGTGAACC	TTGTCCGGTATCCCTTGTCT	50.0	50.0	57.9	58.1	1734-1745 bp	1.0	82.2
ndhF-rpl32_p5	TTTGCAGCAATAGGTCGTGT	TGTCCGGTATCCCTTGTCTT	45.0	50.0	58.1	58.1	1738-1749 bp	1.0	82.1