Hotspot

rps12-clpP

LSC · 71213-71376 · 164 bp

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Pi 0.0793

MarkerSeek score 74.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 13
pi: 0.0793
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGATTGGCTGTCTTGTGTTTC	TGTTGATCTTGTAGCGGTCG	42.9	50.0	57.0	58.0	235-245 bp	1.0	68.4
rps12-clpP_p2	TGATTGGCTGTCTTGTGTTTC	TATGTCAGCAACAGAAGCCC	42.9	50.0	57.0	57.9	268-278 bp	1.0	67.0
rps12-clpP_p3	TGATTGGCTGTCTTGTGTTT	TGTTGATCTTGTAGCGGTCG	40.0	50.0	55.8	58.0	235-245 bp	1.0	66.4
rps12-clpP_p4	TTGATTGGCTGTCTTGTGTT	TGTTGATCTTGTAGCGGTCG	40.0	50.0	55.8	58.0	236-246 bp	1.0	66.4
rps12-clpP_p5	TTTGATTGGCTGTCTTGTGT	TGTTGATCTTGTAGCGGTCG	40.0	50.0	55.8	58.0	237-247 bp	1.0	66.4