Hotspot

psbL-psbF

LSC · 65456-65476 · 21 bp

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Pi 0.0952

MarkerSeek score 77.20

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 21
variable_sites: 2
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	ACGGACTAGCTGTACCTACC	45.0	55.0	58.0	58.0	112 bp	1.0	80.7
psbL-psbF_p2	TCGTTCGGGTTTGATTGTGT	GGACTAGCTGTACCTACCGT	45.0	55.0	58.0	58.0	110 bp	1.0	80.7
psbL-psbF_p3	TCGTTCGGGTTTGATTGTGT	TTCACGGACTAGCTGTACCT	45.0	50.0	58.0	57.8	115 bp	1.0	79.4
psbL-psbF_p4	TCGTTCGGGTTTGATTGTGT	GTTCACGGACTAGCTGTACC	45.0	55.0	58.0	57.7	116 bp	1.0	78.8
psbL-psbF_p5	CGTTCGGGTTTGATTGTGTC	GGACTAGCTGTACCTACCGT	50.0	55.0	57.7	58.0	109 bp	1.0	78.6