Hotspot

trnfM-CAU-rps14

LSC · 37573-37724 · 152 bp

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Pi 0.0662

MarkerSeek score 74.90

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 10
pi: 0.0662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	CAGGGGCAACAAGATCAAGT	50.0	50.0	58.0	58.1	219-220 bp	1.0	83.1
trnfM-CAU-rps14_p2	TTTGAACCCGTGACCTCAAG	CAGGGGCAACAAGATCAAGT	50.0	50.0	58.0	58.1	236-237 bp	1.0	82.7
trnfM-CAU-rps14_p3	AAGGTTATGAGCCTTGCGAG	CACACATGTTTGTTGCCAGG	50.0	50.0	58.0	58.1	235-236 bp	1.0	82.3
trnfM-CAU-rps14_p4	ACAGGATTTGAACCCGTGAC	CAGGGGCAACAAGATCAAGT	50.0	50.0	58.1	58.1	242-243 bp	1.0	82.1
trnfM-CAU-rps14_p5	TTTGAACCCGTGACCTCAAG	CACACATGTTTGTTGCCAGG	50.0	50.0	58.0	58.1	252-253 bp	1.0	82.0