Hotspot

trnY-GUA-trnE-UUC

LSC · 31419-31482 · 64 bp

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Pi 0.0469

MarkerSeek score 70.50

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 3
pi: 0.0469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACATATTGCCA	ATGTATGCCCCCATCGTCTA	50.0	50.0	57.8	58.0	207 bp	1.0	79.1
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACATATTGCC	ATGTATGCCCCCATCGTCTA	50.0	50.0	57.8	58.0	208 bp	1.0	79.0
trnY-GUA-trnE-UUC_p3	CGAATTTACAGTCCGTCCCC	ATGTATGCCCCCATCGTCTA	55.0	50.0	58.4	58.0	186 bp	1.0	78.3
trnY-GUA-trnE-UUC_p4	ACGAATTTACAGTCCGTCCC	ATGTATGCCCCCATCGTCTA	50.0	50.0	57.6	58.0	187 bp	1.0	78.1
trnY-GUA-trnE-UUC_p5	CATCTTATAGCGGATGGGCG	ATGTATGCCCCCATCGTCTA	55.0	50.0	58.4	58.0	253 bp	1.0	77.9