Hotspot

trnD-GUC-trnY-GUA

LSC · 30932-31334 · 403 bp

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Pi 0.0447
MarkerSeek score 69.00
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
403
variable_sites
18
pi
0.0447
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 473-498 bp 1.0 77.2
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 472-497 bp 1.0 77.2
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 494-519 bp 1.0 76.2
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 493-518 bp 1.0 76.0
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.5 58.4 527-552 bp 1.0 75.6