Hotspot

rpoC1-rpoB

LSC · 23975-24000 · 26 bp

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Pi 0.0769

MarkerSeek score 80.90

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 2
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGGCCCAAGCACTTATTTGT	CGACTGCTCGTTCGAGAATT	45.0	50.0	58.0	58.4	195 bp	1.0	77.7
rpoC1-rpoB_p2	TGTGGCCCAAGCACTTATTT	CGACTGCTCGTTCGAGAATT	45.0	50.0	58.0	58.4	197 bp	1.0	77.7
rpoC1-rpoB_p3	TTGTGGCCCAAGCACTTATT	CGACTGCTCGTTCGAGAATT	45.0	50.0	58.0	58.4	198 bp	1.0	77.7
rpoC1-rpoB_p4	TTTGTGGCCCAAGCACTTAT	CGACTGCTCGTTCGAGAATT	45.0	50.0	58.0	58.4	199 bp	1.0	77.7
rpoC1-rpoB_p5	AATTTTTGTGGCCCAAGCAC	CGACTGCTCGTTCGAGAATT	45.0	50.0	58.0	58.4	203 bp	1.0	77.5