Hotspot

rps16-trnQ-UUG

LSC · 6422-7293 · 872 bp

Back to Sesuvium

Pi 0.032

MarkerSeek score 72.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 26
pi: 0.032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	935-966 bp	1.0	84.7
rps16-trnQ-UUG_p2	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	909-940 bp	1.0	84.1
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	925-956 bp	1.0	84.0
rps16-trnQ-UUG_p4	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	965-996 bp	1.0	83.8
rps16-trnQ-UUG_p5	GCACGTTGCTTTTTACCACA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	57.8	58.2	939-970 bp	1.0	83.2