Hotspot

trnH-GUG-psbA

LSC · 166-536 · 371 bp

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Pi 0.0634

MarkerSeek score 84.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 371
variable_sites: 21
pi: 0.0634
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	408-440 bp	1.0	86.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	422-454 bp	1.0	86.2
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	421-453 bp	1.0	86.2
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	423-455 bp	1.0	86.2
trnH-GUG-psbA_p5	TAGTAACTTGGTCTCGGGCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	533 bp	0.5	58.8