Hotspot

rpl16

LSC · 77637-78942 · 1306 bp

Back to Sesleria

Pi 0.00306

MarkerSeek score 60.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1306
variable_sites: 4
pi: 0.00306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCCGTTTTAGTTTTAGGGGG	TTCCTGAAATCCCTGAAAAA	47.6	35.0	57.8	52.5	1415-1416 bp	1.0	40.2
rpl16_p2	CCCGTTTTAGTTTTAGGGGGT	TTCCTGAAATCCCTGAAAAA	47.6	35.0	57.8	52.5	1414-1415 bp	1.0	40.2
rpl16_p3	CCCGTTTTAGTTTTAGGGGGT	TTTCCTGAAATCCCTGAAAA	47.6	35.0	57.8	52.5	1415-1416 bp	1.0	40.2
rpl16_p4	CCCGTTTTAGTTTTAGGGGGT	TTTTCCTGAAATCCCTGAAA	47.6	35.0	57.8	52.5	1416-1417 bp	1.0	40.2
rpl16_p5	CCCGTTTTAGTTTTAGGGGGT	TTTTTCCTGAAATCCCTGAA	47.6	35.0	57.8	52.5	1417-1418 bp	1.0	40.2