Hotspot

ndhF-rpl32

SSC · 104221-105109 · 889 bp

Back to Sesleria

Pi 0.0045
MarkerSeek score 51.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
889
variable_sites
4
pi
0.0045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 1000-1001 bp 1.0 40.3
ndhF-rpl32_p2 GGCCAAACCCCATTGACATA TCCAAATATTTTTACGAATACGCT 50.0 29.2 58.1 55.3 1026-1027 bp 1.0 40.3
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 973-974 bp 1.0 40.3
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 974-975 bp 1.0 40.3
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 975-976 bp 1.0 40.3