Hotspot

psbJ-psbL

LSC · 61554-61678 · 125 bp

Back to Sesleria

Pi 0.024

MarkerSeek score 66.30

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 3
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TCTTCCAGTAGTATCGGCCA	TGACACAATCAAACCCGAATG	50.0	42.9	57.6	57.1	262 bp	1.0	62.0
psbJ-psbL_p2	TCTTCCAGTAGTATCGGCCA	GACACAATCAAACCCGAATGA	50.0	42.9	57.6	56.8	261 bp	1.0	59.8
psbJ-psbL_p3	TCTTCCAGTAGTATCGGCCA	CACAATCAAACCCGAATGAACA	50.0	40.9	57.6	57.8	259 bp	1.0	59.5
psbJ-psbL_p4	TCTTCCAGTAGTATCGGCCA	ACACAATCAAACCCGAATGAAC	50.0	40.9	57.6	57.8	260 bp	1.0	59.4
psbJ-psbL_p5	TCTTCCAGTAGTATCGGCCA	ACTATGACACAATCAAACCCGA	50.0	40.9	57.6	57.7	266 bp	1.0	59.1