Hotspot

petA-psbJ

LSC · 60582-61430 · 849 bp

Back to Sesleria

Pi 0.00236

MarkerSeek score 54.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 2
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	CGGTTCCTATTCTGGATTGGG	50.0	52.4	57.9	58.1	985 bp	1.0	70.6
petA-psbJ_p2	TGTTCTTCTTCGCATCCGTT	CGGTTCCTATTCTGGATTGGG	45.0	52.4	57.8	58.1	978 bp	1.0	69.7
petA-psbJ_p3	TTGTTCTTCTTCGCATCCGT	CGGTTCCTATTCTGGATTGGG	45.0	52.4	57.8	58.1	979 bp	1.0	69.7
petA-psbJ_p4	CGCATCCGTTATTTTGGCAC	CGGTTCCTATTCTGGATTGGG	50.0	52.4	58.5	58.1	968 bp	1.0	67.3
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	ACGGTTCCTATTCTGGATTGG	50.0	47.6	57.9	57.4	986 bp	1.0	67.3