Hotspot

rbcL-psaI

LSC · 55977-57273 · 1297 bp

Back to Sesleria

Pi 0.00926

MarkerSeek score 64.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1297
variable_sites: 12
pi: 0.00926
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AGTTCGAGCCGGTAGATACT	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.7	57.9	1399-1400 bp	1.0	77.4
rbcL-psaI_p2	GGCGATCAAATTCGAGTTCG	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.6	57.9	1413-1414 bp	1.0	77.3
rbcL-psaI_p3	TGGAAGGCGATCAAATTCGA	GTCATTGCAATTGCCGGAAA	45.0	45.0	57.6	57.9	1418-1419 bp	1.0	77.0
rbcL-psaI_p4	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.5	57.9	1461-1462 bp	1.0	76.4
rbcL-psaI_p5	CAGCTTGCAAATGGAGTCCT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.5	57.9	1462-1463 bp	1.0	76.4