Hotspot

ndhC-trnV-UAC

LSC · 50008-50794 · 787 bp

Back to Sesleria

Pi 0.00762
MarkerSeek score 63.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
787
variable_sites
6
pi
0.00762
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCTGAAATCCAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.0 57.9 897 bp 1.0 79.7
ndhC-trnV-UAC_p2 GCCCTGAAATCCAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.0 58.3 898 bp 1.0 77.9
ndhC-trnV-UAC_p3 CTCTGGTCCTTCACTAACCG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.3 57.9 926 bp 1.0 74.7
ndhC-trnV-UAC_p4 TCTGGTCCTTCACTAACCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.7 57.9 925 bp 1.0 74.0
ndhC-trnV-UAC_p5 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 916 bp 1.0 73.7