Hotspot

trnC-GCA-rpoB

LSC · 18283-19449 · 1167 bp

Back to Sesleria

Pi 0.00429

MarkerSeek score 61.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1167
variable_sites: 5
pi: 0.00429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1235-1236 bp	1.0	79.5
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1242-1243 bp	1.0	78.4
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TGTCAAGTTCTTCTGCCAGG	50.0	50.0	58.1	57.7	1318-1319 bp	1.0	77.7
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1250-1251 bp	1.0	77.2
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1249-1250 bp	1.0	77.2