Hotspot

petN-trnC-GCA

LSC · 17304-18211 · 908 bp

Back to Sesleria

Pi 0.00551

MarkerSeek score 62.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 5
pi: 0.00551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	TTTCTCGATTTTCGCTCGGA	50.0	45.0	58.2	57.6	1032 bp	1.0	76.4
petN-trnC-GCA_p2	CCACTCCTCCCCCATACTAC	TTTCTCGATTTTCGCTCGGA	60.0	45.0	58.3	57.6	1088 bp	1.0	75.3
petN-trnC-GCA_p3	TCCACTCCTCCCCCATACTA	TTTCTCGATTTTCGCTCGGA	55.0	45.0	58.4	57.6	1089 bp	1.0	74.6
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	TCTCGATTTTCGCTCGGATT	50.0	45.0	58.2	57.4	1030 bp	1.0	74.6
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	TTCTCGATTTTCGCTCGGAT	50.0	45.0	58.2	57.4	1031 bp	1.0	74.6