Hotspot

trnG-GCC-trnfM-CAU

LSC · 12217-12714 · 498 bp

Back to Sesleria

Pi 0.00602

MarkerSeek score 52.10

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 3
pi: 0.00602
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GTCGACCCAAGCGGATATAC	TTTTTGGTACTCCAGAGCGG	55.0	50.0	57.9	57.8	698-726 bp	1.0	78.4
trnG-GCC-trnfM-CAU_p2	GCGAGCGTAGTTCAATGGTA	TTTTTGGTACTCCAGAGCGG	50.0	50.0	58.1	57.8	658-686 bp	1.0	78.3
trnG-GCC-trnfM-CAU_p3	GTCGACCCAAGCGGATATAC	CGAGGCTCATAACCTTGAGG	55.0	55.0	57.9	57.8	656-684 bp	1.0	78.0
trnG-GCC-trnfM-CAU_p4	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	616-644 bp	1.0	77.9
trnG-GCC-trnfM-CAU_p5	GTCGACCCAAGCGGATATAC	CAGTTTGGTAGCTCACGAGG	55.0	55.0	57.9	58.3	671-699 bp	1.0	77.6