Hotspot

psbC-trnS-UGA

LSC · 11088-11239 · 152 bp

Back to Sesleria

Pi 0.0197

MarkerSeek score 63.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 3
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	341 bp	1.0	80.5
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.0	58.1	333 bp	1.0	80.3
psbC-trnS-UGA_p3	CATTTGTGGCATGCAGGAAG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.0	58.1	334 bp	1.0	80.3
psbC-trnS-UGA_p4	CATTTGTGGCATGCAGGAAG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.0	58.1	326 bp	1.0	80.2
psbC-trnS-UGA_p5	GCTGCAGCAGGTTTTGAAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.1	58.0	311 bp	1.0	80.1