Hotspot

psbI-trnS-GCU

LSC · 7450-7568 · 119 bp

Back to Sesleria

Pi 0.0087
MarkerSeek score 60.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
119
variable_sites
1
pi
0.0087
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 TCTAATGACCCAGGACGGAA TAAAGCGGCGGATTGCTAAT 50.0 45.0 57.8 58.0 222-226 bp 1.0 79.5
psbI-trnS-GCU_p2 ATCTAATGACCCAGGACGGA TAAAGCGGCGGATTGCTAAT 50.0 45.0 57.5 58.0 223-227 bp 1.0 77.7
psbI-trnS-GCU_p3 TCTAATGACCCAGGACGGAA TGATTTGGAACGGGGAGAGA 50.0 50.0 57.8 58.3 255-259 bp 1.0 76.8
psbI-trnS-GCU_p4 TCTAATGACCCAGGACGGAA ATGGCTGAGTGGACTAAAGC 50.0 50.0 57.8 57.6 236-240 bp 1.0 76.5
psbI-trnS-GCU_p5 TCTAATGACCCAGGACGGAA TTGATTTGGAACGGGGAGAG 50.0 50.0 57.8 57.5 256-260 bp 1.0 75.7