Hotspot

ycf1

SSC · 125576-131071 · 5496 bp

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Pi 0.00864
MarkerSeek score 48.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5496
variable_sites
265
pi
0.00864
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GTAGTTCGCCGTTTTTGTGG GCTGAGGACTTCGATGAGTG 50.0 55.0 58.0 58.1 1316-1364 bp 1.0 88.9
ycf1_p2 GTAGTTCGCCGTTTTTGTGG GAAACCTTGGCACAGATCCA 50.0 50.0 58.0 58.1 1179-1227 bp 0.882353 84.2
ycf1_p3 GTAGTTCGCCGTTTTTGTGG CCCTTCCCTTTGGTTCTGTC 50.0 55.0 58.0 58.1 1072-1120 bp 0.882353 83.6
ycf1_p4 GTAGTTCGCCGTTTTTGTGG CCTTCCCTTTGGTTCTGTCC 50.0 55.0 58.0 58.1 1071-1119 bp 0.882353 83.6
ycf1_p5 GTAGTTCGCCGTTTTTGTGG CTTCCCTTTGGTTCTGTCCC 50.0 55.0 58.0 58.1 1070-1118 bp 0.882353 83.6