Hotspot

psbA

LSC · 310-1371 · 1062 bp

Pi 0.00298

MarkerSeek score 40.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 22
pi: 0.00298
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	AAATTCGTGTGCTTGGGAGT	55.0	45.0	58.1	58.0	1325-1366 bp	1.0	86.1
psbA_p2	CAATCCACTGCCTTGATCCA	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.9	58.0	1339-1380 bp	1.0	85.9
psbA_p3	AATCCACTGCCTTGATCCAC	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.9	58.0	1338-1379 bp	1.0	85.9
psbA_p4	ACAATCCACTGCCTTGATCC	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.9	58.0	1340-1381 bp	1.0	85.9
psbA_p5	GATCCACTTGGCTACATCCG	AAAGCTTCTTCCACTTCGCA	55.0	45.0	58.1	58.0	1861-1904 bp	0.764706	76.4