Hotspot

rps19-rpl2

LSC · 84672-84728 · 57 bp

Back to Seseli

Pi 0.0337
MarkerSeek score 77.40
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
LSC
length_bp
57
variable_sites
7
pi
0.0337
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 AGATGCCCGAGACCAAGTTA GGTTATCCTGCACTTGGGAG 50.0 55.0 58.4 58.0 250-261 bp 1.0 82.2
rps19-rpl2_p2 TAGATGCCCGAGACCAAGTT GGTTATCCTGCACTTGGGAG 50.0 55.0 58.4 58.0 251-262 bp 1.0 82.1
rps19-rpl2_p3 AGATGCCCGAGACCAAGTTA CCTGCACTTGGGAGAAGAAG 50.0 55.0 58.4 58.2 244-255 bp 1.0 81.2
rps19-rpl2_p4 AGATGCCCGAGACCAAGTTA CACAACCCCTTGGGGTTATC 50.0 55.0 58.4 58.2 263-274 bp 1.0 81.0
rps19-rpl2_p5 TAGATGCCCGAGACCAAGTT CACAACCCCTTGGGGTTATC 50.0 55.0 58.4 58.2 264-275 bp 1.0 81.0