Hotspot

psbE-petL

LSC · 65273-66277 · 1005 bp

Back to Seseli

Pi 0.00944
MarkerSeek score 69.40
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1005
variable_sites
55
pi
0.00944
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1080-1176 bp 1.0 87.4
psbE-petL_p2 ACTGACGAATAACCAACCCG GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1093-1189 bp 1.0 87.0
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1083-1179 bp 1.0 86.9
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.8 58.0 1003-1099 bp 1.0 86.8
psbE-petL_p5 ACTGACGAATAACCAACCCG TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1096-1192 bp 1.0 86.5