Hotspot

petA-psbJ

LSC · 63537-64491 · 955 bp

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Pi 0.0184
MarkerSeek score 67.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
955
variable_sites
83
pi
0.0184
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTCGAGAAGGTTCA CTGGATTGGGTTCATCTCTGT 40.9 47.6 57.6 57.4 703-1094 bp 1.0 58.4
petA-psbJ_p2 TCGAGAAGGTTCAATTGTCAGA CTGGATTGGGTTCATCTCTGT 40.9 47.6 57.4 57.4 694-1085 bp 1.0 56.9
petA-psbJ_p3 AGAAACAGTTCGAGAAGGTTCA TGGATTGGGTTCATCTCTGT 40.9 45.0 57.6 56.1 702-1093 bp 1.0 56.5
petA-psbJ_p4 AGAAACAGTTCGAGAAGGTTCA TCTGGATTGGGTTCATCTCTG 40.9 47.6 57.6 57.1 704-1095 bp 1.0 56.1
petA-psbJ_p5 TCGAGAAGGTTCAATTGTCAGA TGGATTGGGTTCATCTCTGT 40.9 45.0 57.4 56.1 693-1084 bp 1.0 55.0