Hotspot

trnT-GGU-psbD

LSC · 32218-33681 · 1464 bp

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Pi 0.0153
MarkerSeek score 74.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1464
variable_sites
109
pi
0.0153
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AAGGGCCCTTTTAACTCAGC AGACCGGACCATCCTACAAA 50.0 50.0 58.1 58.1 1259-1648 bp 1.0 88.7
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA AGACCGGACCATCCTACAAA 50.0 50.0 57.9 58.1 1255-1644 bp 1.0 88.4
trnT-GGU-psbD_p3 AAGGGCCCTTTTAACTCAGC GAGCAATAGACCGGACCATC 50.0 55.0 58.1 57.8 1266-1655 bp 1.0 87.8
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA GAGCAATAGACCGGACCATC 50.0 55.0 57.9 57.8 1262-1651 bp 1.0 87.6
trnT-GGU-psbD_p5 AAGGGCCCTTTTAACTCAGC AGAGCAATAGACCGGACCAT 50.0 50.0 58.1 58.2 1267-1656 bp 1.0 87.4