Hotspot

atpH-atpI

LSC · 13516-14649 · 1134 bp

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Pi 0.0115
MarkerSeek score 69.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1134
variable_sites
83
pi
0.0115
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT ATAGGGGAATCCATGGAGGG 45.0 55.0 58.0 57.9 1195-1260 bp 1.0 86.2
atpH-atpI_p2 TACCTTGACCAACTCCAGGT ATAGGGGAATCCATGGAGGG 50.0 55.0 57.9 57.9 1251-1316 bp 1.0 85.5
atpH-atpI_p3 AGCCAATCCAGCAGCAATAA ATAGGGGAATCCATGGAGGG 45.0 55.0 57.9 57.9 1211-1276 bp 1.0 85.3
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA ATAGGGGAATCCATGGAGGG 45.0 55.0 58.2 57.9 1196-1261 bp 1.0 85.1
atpH-atpI_p5 GCAGTACCTTGACCAACTCC ATAGGGGAATCCATGGAGGG 55.0 55.0 58.2 57.9 1255-1320 bp 0.941176 79.6